- 产品描述
OXOID C. DIFFICLE TOXIN A STOOL FILTRATION SYSTEM
Code: TD0975A
INTENDED USE
The Oxoid stool filtration system is an alternative preparation technique for the isolation of C. difficletoxin A from stool samples prior to testing with the Oxoid C. difficle Toxin A Test (TD0970A). The system is intended exclusively for for use with the Oxoid C. difficle Toxin A Test and does not require the use of a centrifuge.
SAMPLE PREPARATION
1. Note: All components and reagents should be allowed to reach room temperature (18-30° C) for 30 minutes before use.
2. a) Liquid Stools - Pippette 1ml of sample diluent to the sample cup of the stool filtration system. Using a pipette, add 100m of stool to the sample diluent in the the sample cup.
b) Solid or Semi-Solid Stools - Pippette 1ml of sample diluent to the sample cup. Using a loop, add a 5mm diameter bead-like portion of stool into the sample cup.
3. Mix the sample thoroughly using a pipette or loop, as appropriate, to form an even suspension.
4. Place the filter body (with cap in place) into the sample cup, and push downward on the cap so that the filter body stops approximay 2mm above the level of the liquid.
5. Allow the particulate matter to settle by standing the stool filter system on a level surface for 1 minute.
6. Apply further pressure to the cap of the filter body, and continue to push downwards so that the sample passes through the filter into the filter body.
TEST PROCEDURE
Remove the toxin test unit from the foil puch and place on a level surface. Remove the cap from the body of the filter unit. Invert the stool filtration system and apply 6 drops of the stool filtrate to the sample window of the C. difficle Toxin A Test unit, by squeezing the sides of the nozzle.
INTERPRETATION OF RESULTS
Following application of sample from the stool filtration system to the test device results should be interpreted as directed in the C. difficle Toxin A Test product insert.