PCR试剂 细菌性肠胃炎的9种菌属PCR核酸检测试剂盒

广州健仑生物科技有限公司

两种准备使用lyo master混合物（各8孔条），用于检测结肠弯曲杆菌/空肠弯曲菌/红嘴鸥弯曲杆菌，艰难梭菌，大肠杆菌Verooxin阳性，沙门氏菌属，志贺氏菌属，肠侵袭性大肠杆菌，小肠结肠炎耶尔森氏菌包括内部对照。
Two ready to use lyo master mixes (8-well strips each) for detection of Campylobacter coli/jejuni /lari, Clostridium difficile, Escherichia coli verotoxin positives, Salmonella spp., Shigella spp., enteroinvasive Escherichia coli, Yersinia enterocolitica and internal control

PCR试剂 细菌性肠胃炎的9种菌属PCR核酸检测试剂盒

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PCR试剂

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二、消除非特异性染色的方法 
消除荧光抗体非特异性染色的方法应根据产生的原因采取适当的方法，常用的方法有以下几种： 
（一）动物脏器粉末吸收法 
常用肝粉（猪、大白鼠或小白鼠），其次是骨髓粉、鼠脑粉和鸡胚粉等。每毫升荧光抗体中加入肝粉50～100mg,在离心管中充分混匀，在室温中振动2h，4℃中过夜，再搅拌10min，高速离心（3000～15000r/min）30min，1～2次后，即可使用其上清液。吸收一般应在临用前进行，吸收后之荧光抗体保存冰箱中勿超过2周。染色应作吸收前后之比较，吸收时可先用缓冲盐水将组织干粉浸湿，离心（3000～15000r/min）30min，除去上清液，再加入荧光抗体进行吸收，以免消耗过多的抗体。 
肝粉或新鲜细胞吸收是一种非特异性的消除方法，对荧光抗体的荧光色素和蛋白都有吸附作用。如检查组织中的病毒抗原时，也可用相同的组织干粉或匀浆沉淀物吸收之。 
用脏器肝粉吸收对荧光抗体损失较多，如果根据Hiramotos氏等的方法将组织的20%生理盐水匀浆液，用生理盐水洗2～3次，12000r/min  10min离心沉淀，用其沉淀物吸收其荧光抗体即能*达到目的，京极方久氏认为这样吸收对荧光抗体几乎没有损失，他们常用此法，效果甚佳，吸收后放置一周左右，用时有必要再吸收一次。 

Second, to eliminate non-specific staining methods
Elimination of non-specific fluorescent antibody staining method should be based on the reasons for taking the appropriate method, commonly used methods are the following:
(A) animal organ powder absorption method
Commonly used liver powder (pigs, rats or mice), followed by bone marrow powder, rat brain and chicken embryo powder and so on. Add 50 ~ 100mg of liver powder per ml of fluorescent antibody, mix thoroughly in a centrifuge tube, shake at room temperature for 2h, overnight at 4 ℃, stir for 10min, centrifuge at high speed (3000 ~ 15000r / min) for 30min, After that, you can use the supernatant. Absorption generally should be carried out immediay before use, after the absorption of fluorescent antibodies stored in the refrigerator for not more than 2 weeks. Dyeing should be compared before and after absorption, the absorption can be washed with saline buffer wet tissue, centrifugation (3000 ~ 15000r / min) 30min, remove the supernatant, then add fluorescent antibodies to absorb, so as not to consume too much antibody.
Liver powder or fresh cell absorption is a nonspecific method of elimination, the fluorescence of fluorescent antibodies and proteins have adsorption. If you check the virus antigen in your tissue, you can also use the same tissue dry powder or homogenate sediment absorption.
With the organism liver powder absorption of fluorescent antibody loss more, according to Hiramotos's method of tissue 20% normal saline homogenate, washed with saline 2-3 times, 12000r / min 10min centrifugal precipitation, with its precipitate Absorption of its fluorescent antibodies that can fully achieve the purpose, Kyrgyzstan side Kyrgyzstan think this absorption of almost no loss of fluorescent antibodies, they commonly used this method, the effect is very good, after absorption for a week or so, when necessary it is necessary to absorb again.